Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label.By extracting DNA from a complete adipose tissue depot surface awaken protein mousse stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the number of adipocytes can be determined based on the bond no.9 nuits de noho quantification of the recombined LoxPRed sites.This highly sensitive system allows for the quantification of white, brown, and brite/beige adipocytes in a spatially unbiased and size-independent manner.For complete details on the use and execution of this protocol, please refer to Moser et al.(2021).